GMP Human IL-7
Catalog No: IL7-HP002
- Species
- Human
- Expression System
- E. coli
- Activity
- Activity verified
Product overview
Recombinant GMP Human IL-7 is expressed in E. coli without tag. It contains amino acid residues Asp26-His177 (UniProt accession: P13232).
Product Details
- Molecular Aliases
- IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF
- Protein Length
- Asp26-His177
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 17.5 kDa same as Bis-Tris PAGE result.
- Purity
- ≥ 95% as determined by SEC-HPLC.
- Endotoxin
- ≤ 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- The product is shipped with ice bag. Store it immediately at -20°C±5°C upon arrival. -20°C±5°C for 36 months in lyophilized state. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
GMP Human IL-7 on Bis-Tris PAGE under reduced condition.
Measured in a cell proliferation assay using PHA-activated human peripheral blood mononuclear cell (PBMC). The specific activity of GMP Human IL-7 is > 1 × 10^8 IU/mg, which is calibrated against the human IL-7 WHO reference standard (NIBSC code: 90/530) (QC Test).
Background
Interleukin (IL)-7 is a non-redundant cytokine in T-cell development and function. It is required in early T-cell development as well as for T-cell homeostasis and is secreted by stromal cells in the thymic and bone marrow (BM) environment. IL-7 binds to its receptor which is composed of the two chains IL-7Rα (CD127), shared with the thymic stromal lymphopoietin (TSLP), and the common γ chain (CD132) for IL-2, IL-15, IL-9 and IL-21. Whereas γc is expressed by most hematopoietic cells, IL-7Rα is nearly exclusively expressed on lymphoid cells. After binding to its receptor, IL-7 signals through two different pathways: Jak-Stat (Janus kinase-Signal transducer and activator of transcription) and PI3K/Akt responsible for differentiation and survival, respectively.
References
- ElKassar N, Gress RE. An overview of IL-7 biology and its use in immunotherapy. J Immunotoxicol. 2010;7(1):1-7. doi:10.3109/15476910903453296
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