Cynomolgus Fc gamma RIIA/CD32a Protein
Catalog No: CD32A-CP001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Gln28-Pro208 (UniProt accession: Q8SPW4-1).
Product Details
- Molecular Aliases
- Fc gamma RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; FCG2; CD32A; FcγRIIA; Fc γRIIA; Fcγ RIIA; Fc γ RIIA
- Protein Length
- Gln28-Pro208
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Cynomolgus Fc gamma RIIA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus Fc gamma RIIA is greater than 95% as determined by SEC-HPLC.
Cynomolgus Fc gamma RIIA, His Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 4.39 μM as determined in SPR assay (Biacore T200). (QC Test)
Loaded Cynomolgus Fc gamma RIIA, His Tag on Anti-His-Biosensor, can bind Rituximab with an affinity constant of 1.75 µM as determined in BLI assay (Gator).
Background
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
References
- Brooks, D. G. Structure and expression of human IgG FcRII(CD32). Functional heterogeneity is encoded by the alternatively spliced products of multiple genes[J]. Journal of Experimental Medicine, 1989, 170(4):1369-1385.
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