Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A3 (KVAELVHFL) Monomer Protein
Catalog No: MAGEA3-HP005
- Species
- Human&Mouse
- Expression System
- HEK293
- Tag
- His
Product overview
Recombinant Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A3 (KVAELVHFL) Monomer Protein is expressed from HEK293 with His tag at the C-terminus. It contains Gly25-Thr206 (Human HLA-A*02:01 α1&α2) and Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and KVAELVHFL peptide (accession: A0A140T913(Human HLA-A*02:01 α1 & α2) & P01897(Mouse H-2Ld α3) & P01887(Mouse B2M) &KVAELVHFL).
Product Details
- Molecular Aliases
- MAGE-A3
- Protein Length
- Gly25-Thr206
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 48.00 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A3 (KVAELVHFL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A3 (KVAELVHFL) Monomer is greater than 95% as determined by SEC-HPLC.
Background
Melanoma antigen family A, 3 (MAGE-A3) is a cancer-testis antigen whose expression has been demonstrated in a wide array of malignancies including melanoma, brain, breast, lung and ovarian cancer. In addition, its ability to elicit spontaneous humoral and cellular immune responses has been shown in cancer patients.
References
- Esfandiary A, Ghafouri-Fard S. MAGE-A3: an immunogenic target used in clinical practice. Immunotherapy. 2015;7(6):683-704. doi: 10.2217/imt.15.29. Epub 2015 Jun 23. PMID: 26100270.
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