Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer Protein
Catalog No: MAGEA3-HB001
- Species
- Human
- Expression System
- E. coli
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*01:01), Ile21-Met119(B2M) and EVDPIGHLY peptide (accession: Q5SUL5(HLA-A*01:01) & P61769(B2M) &EVDPIGHLY).
Product Details
- Molecular Aliases
- Melanoma-associated antigen 3; MZ2-D; MZ2D; MZ2-D; CT1.3; MAGE-3
- Protein Length
- Gly25-Thr305
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.84 kDa (HLA-A*01:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer is greater than 95% as determined by SEC-HPLC.
Background
Melanoma antigen gene A3 (MAGE-A3) is one of the most immunogenic cancer testis antigens and is common in various types of cancers. MAGE-A3 can be considered as a predictor for poor prognosis and an option for vaccine immunotherapy in patients with PCa.
References
- Khalvandi A, Abolhasani M, Madjd Z, Shekarabi M, Kourosh-Arami M, Mohsenzadegan M. Nuclear overexpression levels of MAGE-A3 predict poor prognosis in patients with prostate cancer. APMIS. 2021 Jun;129(6):291-303. doi: 10.1111/apm.13132. Epub 2021 Apr 13. PMID: 33743542.
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