PROVETOP

Biotinylated SARS-COV-2 Nucleocapsid Protein CTD Domain Protein

Catalog No: SARS-HB009

Species
SARS-COV-2
Expression System
E.coli
Tag
His, Avi

Product overview

Recombinant Biotinylated SARS-CoV-2 Nucleocapsid Protein CTD Domain Protein is expressed in E. coli with a His tag and Avi tag at the N-terminus. It contains amino acid residues Gly243-Pro364 (accession: QHD43423.2).

Product Details

Molecular Aliases
Nucleocapsid; NP; NC
Protein Length
Gly243-Pro364
Expression System
E.coli
Theoretical Molecular Weight
The protein has a predicted MW of 17 kDa same as Bis-Tris PAGE result.
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per μg by the LAL method.
Buffer / Formulation
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State
Lyophilized
Storage Conditions
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution Advice
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data Display

Bis-Tris PAGE
SARS-HB009 Bis-Tris-PAGE-white result

Biotinylated SARS-CoV-2 Nucleocapsid CTD Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Background

Nucleocapsid protein (N) is the major viral structural component; its main function is to protect and encapsidate the viral RNA forming viral RNP complex. It is encoded by the S segment vRNA and is abundantly expressed in the cytoplasm of infected cells.

References

  1. Longhi S. Nucleocapsid structure and function. Curr Top Microbiol Immunol. 2009;329:103‐128. doi:10.1007/978-3-540-70523-9_6

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