Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein
Catalog No: SARS-HB003
- Species
- SARS-COV-2
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated SARS-CoV-2 Spike S (Omicron B.1.1.529) Trimer Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F) trimer design. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively (accession: QHO60594.1).
Product Details
- Molecular Aliases
- S protein; Spike glycoprotein; S glycoprotein; COVID-19; COVID19
- Protein Length
- Val16-Pro1213
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 136.70 kDa. Due to glycosylation, the protein migrates to 140-180 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated SARS-CoV-2 Spike S (Omicron B.1.1.529) Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated SARS-CoV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag at 2μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.
Background
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
References
- Parsons, Lisa, Bouwman, Kim M, Azurmendi, Hugo F. Glycosylation of the viral attachment protein of avian coronavirus is essential for host cell and receptor binding[J]. Journal of Biological Chemistry.
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