Biotinylated Mouse PLAU/uPA Protein (active form)
Catalog No: PLAU-MB001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Mouse PLAU/uPA Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly21-Phe433 (UniProt accession: P06869).
Product Details
- Molecular Aliases
- PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD
- Protein Length
- Gly21-Phe433
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 49.00 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 20mM PB, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in 20mM PB (pH 6.0). Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Mouse PLAU on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse PLAU is greater than 90% as determined by SEC-HPLC.
Mouse uPAR isoform 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse PLAU, His Tag with an affinity constant of 0.33 μM as determined in SPR assay (Biacore T200).
Background
Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.
References
- Chen G, Sun J, Xie M, Yu S, Tang Q, Chen L. PLAU Promotes Cell Proliferation and Epithelial-Mesenchymal Transition in Head and Neck Squamous Cell Carcinoma. Front Genet. 2021 May 20;12:651882. doi: 10.3389/fgene.2021.651882. PMID: 34093649; PMCID: PMC8173099.
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