Biotinylated Mouse IL-5 Protein
Catalog No: IL5-MB001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Mouse IL-5 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Met21-Gly133 (UniProt accession: P04401).
Product Details
- Molecular Aliases
- Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF;IL5;IL 5; Interleukin 5
- Protein Length
- Met21-Gly133
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 16.03 kDa. Due to glycosylation, the protein migrates to 16 kDa and 20-35 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per ug by the LAL method.
- Buffer / Formulation
- Supplied as 0.22μm filtered solution in 20mM Tris, 500mM NaCl, 10% Glycerol (pH 7.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Mouse IL-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Mouse IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-5, His-Avi tag with an affinity constant of 0.511 μM as determined in SPR assay (Biacore T200).
Background
IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).
References
- Hassani M, Koenderman L. Immunological and hematological effects of IL-5(Rα)-targeted therapy: An overview. Allergy. 2018 Oct;73(10):1979-1988. doi: 10.1111/all.13451. Epub 2018 Oct 8. PMID: 29611207; PMCID: PMC6220846.
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