Biotinylated Human IL-5 R alpha/CD125 Protein
Catalog No: IL5-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-5 R alpha/CD125 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Asp21-Glu335 (UniProt accession: Q01344-1).
Product Details
- Molecular Aliases
- CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560;IL5R alpha; IL5R subunit alpha
- Protein Length
- Asp21-Glu335
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 38.7 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per ug by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-5 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-5 R alpha is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-5, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.15μg/ml determined by ELISA.
Background
Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology. This is the receptor for interleukin-5. The alpha chain binds to IL5.
References
- Garcia G, Taille C, Laveneziana P, et al. Anti-interleukin-5 therapy in severe asthma[J]. European Respiratory Review, 2013, 22(129):251-257.
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