Biotinylated Mouse IL-23 alpha/IL-23 P19 Protein
Catalog No: IL23-MB001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Mouse IL-23 alpha/IL-23 P19 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Val22-Ala196 (UniProt accession: Q9EQ14).
Product Details
- Molecular Aliases
- Interleukin-23 subunit alpha;IL-23 subunit alpha;IL-23-A;Interleukin-23 subunit p19;IL-23p19;IL23p19;IL23A;P19;SGRF;IL23 subunit alpha
- Protein Length
- Val22-Ala196
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 23.41 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Mouse IL-23 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse IL-23 alpha is greater than 90% as determined by SEC-HPLC.
Background
The heterodimeric cytokine interleukin-23 (IL-23 or IL23A/IL12B) is produced by dendritic cells and macrophages and promotes the proinflammatory and regenerative activities of T helper 17 (Th17) and innate lymphoid cells. A recent study has reported that IL-23 is also secreted by lung adenoma cells and generates an inflammatory and immune-suppressed stroma.
References
- Lim KS, Yong ZWE, Wang H, Tan TZ, Huang RY, Yamamoto D, Inaki N, Hazawa M, Wong RW, Oshima H, Oshima M, Ito Y, Voon DC. Inflammatory and mitogenic signals drive interleukin 23 subunit alpha (IL23A) secretion independent of IL12B in intestinal epithelial cells. J Biol Chem. 2020 May 8;295(19):6387-6400. doi: 10.1074/jbc.RA120.012943. Epub 2020 Mar 24. PMID: 32209656; PMCID: PMC7212629.
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