Biotinylated Human IL-23 alpha&Mouse IL-12 beta Protein
Catalog No: IL23-HB002
- Species
- Human&Mouse
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-23 alpha&Mouse IL-12 beta Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus of IL-23 alpha. It contains amino acid residues Arg20-Pro189 (Human IL-23 alpha) and Met23-Ser335 (Mouse IL-12 beta) (accession: Q9NPF7(Human IL-23 alpha) & P43432-1(Mouse IL-12 beta)).
Product Details
- Molecular Aliases
- IL23 alpha; IL12 beta; IL23 alpha&IL12 beta; IL12 β; IL23 alpha&IL12 β;IL12 b; IL12b; IL23 alpha&IL12 b; IL23 alpha&IL12b
- Protein Length
- Arg20-Pro189
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 21.7 kDa (Human IL-23 alpha) & 35.8 kDa (Mouse IL-12 beta). Due to glycosylation, the protein migrates to 22-25 kDa (Human IL-23 alpha) & 45-60 kDa (Mouse IL-12 beta) based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-23 alpha&Mouse IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-23 alpha&Mouse IL-12 beta is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Human IL-23 alpha & Mouse IL-12 beta, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 26.5ng/ml determined by ELISA. (QC Test)
Immobilized Biotinylated Human IL-23 alpha&Mouse IL-12 beta, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Risankizumab, hFc Tag with the EC50 of 1.3ng/ml determined by ELISA.
Background
Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.
References
- Esch A, Masiarz A, Mossner S, Moll JM, Grötzinger J, Schröder J, Scheller J, Floss DM. Deciphering site 3 interactions of interleukin 12 and interleukin 23 with their cognate murine and human receptors. J Biol Chem. 2020 Jul 24;295(30):10478-10492. doi: 10.1074/jbc.RA120.013935. Epub 2020 Jun 9. PMID: 32518162; PMCID: PMC7383385.
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