Biotinylated Human OX40/TNFRSF4/CD134 Protein
Catalog No: OX40-HB004
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human OX40/TNFRSF4/CD134 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Leu29-Ala216 (UniProt accession: P43489).
Product Details
- Molecular Aliases
- CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue; TXGP1L; IMD16
- Protein Length
- Leu29-Ala216
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human OX40 is greater than 95% as determined by SEC HPLC.
Immobilized Biotinylated Human OX40, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test).
Biotinylated Human OX40, His Tag immobilized on CM5 Chip can bind Human OX40 Ligand, hFc Tag with an affinity constant of 23.48 nM as determined in SPR assay (Biacore T200).
Background
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.
References
- Al-Shamkhani A, Mallett S, Brown M H, et al. Affinity and Kinetics of the Interaction between Soluble Trimeric OX40 Ligand, a Member of the Tumor Necrosis Factor Superfamily, and Its Receptor OX40 on Activated T Cells[J]. Journal of Biological Chemistry, 1997, 272(8):5275-5282. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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