Biotinylated Human OX40 Ligand/TNFSF4 Trimer Protein (Primary Amine Labeling)
Catalog No: OX40-HB005
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Flag
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human OX40 Ligand/TNFSF4 Trimer Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag and Flag tag at the N-terminus. It contains amino acid residues Gln51-Leu183 trimer design (UniProt accession: P23510-1).
Product Details
- Molecular Aliases
- OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L; TXGP1; Glycoprotein Gp34
- Protein Length
- Gln51-Leu183
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 49.7 kDa. Due to glycosylation, the protein migrates to 65-160 kDa based on Bis-Tris PAGE result.
- Purity
- > 90% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human OX40 Ligand Trimer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 90%.
The purity of Biotinylated Human OX40 Ligand Trimer is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Human OX40 Ligand (Trimer), His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human OX40, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA. (QC Test)
Background
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.
References
- Al-Shamkhani A, Mallett S, Brown M H, et al. Affinity and Kinetics of the Interaction between Soluble Trimeric OX40 Ligand, a Member of the Tumor Necrosis Factor Superfamily, and Its Receptor OX40 on Activated T Cells[J]. Journal of Biological Chemistry, 1997, 272(8):5275-5282.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.