Biotinylated Human NKG2A&CD94 Protein
Catalog No: NKG2A-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human NKG2A&CD94 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus of NKG2A and Flag tag at the N-terminus of CD94. It contains amino acid residues Arg100-Leu233(NKG2A) and Ser34-Ile179(CD94) (accession: P26715-1(NKG2A) & Q13241-1(CD94)).
Product Details
- Molecular Aliases
- CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
- Protein Length
- Arg100-Leu233
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 17.9 kDa (CD94)&18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human NKG2A&CD94, His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test).
Background
The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
References
- Hò GT, Celik AA, Huyton T, Hiemisch W, Blasczyk R, Simper GS, Bade-Doeding C. NKG2A/CD94 Is a New Immune Receptor for HLA-G and Distinguishes Amino Acid Differences in the HLA-G Heavy Chain. Int J Mol Sci. 2020 Jun 19;21(12):4362. doi: 10.3390/ijms21124362. PMID: 32575403; PMCID: PMC7352787.
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