Biotinylated Human NKG2A/CD159a Protein
Catalog No: NKG2A-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human NKG2A/CD159a Protein is expressed in HEK293 cells with a His tag and Avi tag at N-terminus. It contains amino acid residues Arg100-Leu233 (UniProt accession: P26715-1).
Product Details
- Molecular Aliases
- NKG2A; NKG2-A; CD94; KLRD1; KP43; KLRC1; CD159A
- Protein Length
- Arg100-Leu233
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 18.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human NKG2A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated Human NKG2A, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA.
Background
NKG2, also known as NKG2A(CD159A), is a member of the killer cell lectin-like receptor family. NKG2A marks a unique immune effector subset preferentially co-expressing the tissue-resident CD103 molecule, but not immune checkpoint inhibitors. It is expressed only in NK-cells, but not in T-cells or B-cells.
References
- Vivier E. Anti-NKG2A mAb is a checkpoint inhibitor that promotes anti-tumor immunity by unleashing both T and NK cells [J]. Cell. Volume 175, Issue 7, 13 December 2018, Pages 1731-1743.e13
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