Biotinylated Human IL-17A/CTLA-8 Protein
Catalog No: CTLA8-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- an Avi, His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-17A/CTLA-8 Protein is expressed in HEK293 cells with an Avi tag and His tag at the C-terminus. It contains amino acid residues Gly24-Ala155 (UniProt accession: Q16552-1).
Product Details
- Molecular Aliases
- CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A;CTLA8; IL17CTLA8;Interleukin-17; Interleukin 17
- Protein Length
- Gly24-Ala155
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 16-30 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-17A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-17A is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Human IL-17A, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human IL-17A, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
Human IL-17R alpha, hFc Tag captured on CM5 Chip via protein A can bind Biotinylated Human IL-17A, His-Avi tag with an affinity constant of 2.18 nM as determined in SPR assay (Biacore T200).
Background
Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
References
- Gaffen S L. An overview of IL-17 function and signaling[J]. Cytokine, 2008, 43(3):0-407.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.