Biotinylated Cynomolgus IL-17A/CTLA-8 Protein
Catalog No: CTLA8-CB001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Cynomolgus IL-17A/CTLA-8 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Gly24-Ala155 (accession: XP_005552816.3).
Product Details
- Molecular Aliases
- CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A;CTLA8; IL17CTLA8;Interleukin-17; Interleukin 17
- Protein Length
- Gly24-Ala155
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 18.02 kDa. Due to glycosylation, the protein migrates to 20-24 kDa, 26-30 kDa and 30-38 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Cynomolgus IL-17A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus IL-17A is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Cynomolgus IL-17A, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 5.6ng/ml determined by ELISA. (QC Test)
Immobilized Biotinylated Cynomolgus IL-17A, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
Background
Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
References
- Gaffen S L. An overview of IL-17 function and signaling[J]. Cytokine, 2008, 43(3):0-407.
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