Biotinylated Human IL-1 Rrp2/IL-1 R6 Protein
Catalog No: IL1-HB003
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-1 Rrp2/IL-1 R6 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Asp20-Arg335 (UniProt accession: Q9HB29-1).
Product Details
- Molecular Aliases
- IL-1 R6; IL-1 Rrp2; IL1RL2; IL1Rrp2; IL1RRP2; IL-36R;IL1R6; IL36R
- Protein Length
- Asp20-Arg335
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 39.1 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human IL-1 Rrp2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-1 Rrp2 is greater than 90% as determined by SEC-HPLC.
Immobilized Biotinylated Human IL-1 Rrp2, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Spesolimab, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. (QC Test)
Background
The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9). IL1RL2 is a receptor for interleukin-36 (IL36A, IL36B and IL36G). After binding to interleukin-36 associates with the coreceptor IL1RAP to form the interleukin-36 receptor complex which mediates interleukin-36-dependent activation of NF-kappa-B, MAPK and other pathways.
References
- Mahil S K, Catapano M, Di Meglio P, et al. An analysis of IL-36 signature genes and individuals with\r, IL1RL2\r, knockout mutations validates IL-36 as a psoriasis therapeutic target[J]. Science Translational Medicine, 2017, 9(411):eaan2514.D
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