Biotinylated Mouse IL-1 Beta/IL-1F2 Protein
Catalog No: IL1-MB001
- Species
- Mouse
- Expression System
- E.coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Mouse IL-1 Beta/IL-1F2 Protein is expressed in E. coli with a His tag and Avi tag at the N-terminus. It contains amino acid residues Val118-Ser269 (UniProt accession: P10749).
Product Details
- Molecular Aliases
- Interleukin-1 beta; IL-1 beta; IL1F2; IL1B; IL-1BETA; IL1F2; IL-1β; IL-1 beta; IL-1B;Interleukin-1 β; IL-1 β; IL-1β; IL-1 β;IL1 beta; IL1BETA; IL1β; IL1 β;Interleukin-1 b; Interleukin-1b; IL-1 b
- Protein Length
- Val118-Ser269
- Expression System
- E.coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 20.30 kDa. The protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Mouse IL-1 Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse IL-1 Beta is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Mouse IL-1 Beta, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-1 R2, hFc Tag with the EC50 of 25.4ng/ml determined by ELISA (QC Test).
Immobilized Mouse IL-1R2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse IL-1 Beta, His Tag with the EC50 of 1.2μg/ml determined by ELISA.
Background
Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells.
References
- Bent R, Moll L, Grabbe S, Bros M. Interleukin-1 Beta-A Friend or Foe in Malignancies? Int J Mol Sci. 2018 Jul 24;19(8):2155. doi: 10.3390/ijms19082155. PMID: 30042333; PMCID: PMC6121377.
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