Biotinylated Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer Protein
Catalog No: HLAA0201-HB007
- Species
- Human
- Expression System
- E. coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and FIFPPSDEQL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &FIFPPSDEQL).
Product Details
- Molecular Aliases
- FIFPPSDEQL
- Protein Length
- Gly25-Thr305
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) Monomer, His Tag at 1μg/ml (100 μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 27.4ng/ml determined by ELISA.
Background
IGKC (Immunoglobulin kappa constant) is a segment of the kappa light chain. This region is important for the stability and function of the antibody molecule, as it helps in the proper folding and assembly of the immunoglobulin. Additionally, the kappa constant region may also be involved in the interaction between the antibody and other immune system components.
References
- [1] Bengtén E, Wilson M, Miller N, Clem LW, Pilström L, Warr GW. Immunoglobulin isotypes: structure, function, and genetics. Curr Top Microbiol Immunol. 2000;248:189-219. doi: 10.1007/978-3-642-59674-2_9. PMID: 10793479.
- [2] Li M, Feng DY, Ren W, Zheng L, Zheng H, Tang M, Cao Y. Expression of immunoglobulin kappa light chain constant region in abnormal human cervical epithelial cells. Int J Biochem Cell Biol. 2004 Nov;36(11):2250-7. doi: 10.1016/j.biocel.2004.03.017. PMID: 15313470.
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