Biotinylated Human GPC3/Glypican 3 Protein
Catalog No: GPC3-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human GPC3/Glypican 3 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln25-His559 (UniProt accession: P51654-1).
Product Details
- Molecular Aliases
- GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1
- Protein Length
- Gln25-His559
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 63.6 kDa. Due to glycosylation, the protein migrates to 30-31 kDa, 38-42 kDa and 65-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human GPC3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human GPC3 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human GPC3, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test).
Use Biotinylated Human GPC3-His protein to detect the expression rate of Anti-GPC3-CAR positive cells. 293T cells and anti-GPC3 CAR-293T cells were incubated with Biotinylated human GPC3-His (25-559), His Tag and Biotinylated Protein Control-His. Non-transfected 293 cells and Biotinylated Protein Control-His were used as negative control. SA-FITC was used to evaluate the binding activity of Biotinylated Human GPC3.
Background
Glypican-3 is a protein,which is encoded by the GPC3 gene in humans. The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa. Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
References
- Ho M, Kim H. Glypican-3: A new target for cancer immunotherapy[J]. European Journal of Cancer, 2011, 47(3):0-338.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.