Biotinylated Human GFRAL/GFR alpha-like Protein
Catalog No: GFRAL-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human GFRAL/GFR alpha-like Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ser19-Glu351 (UniProt accession: Q6UXV0).
Product Details
- Molecular Aliases
- GFR alpha-like; GFRAL; GRAL; C6orf144
- Protein Length
- Ser19-Glu351
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human GFRAL on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human GFRAL is greater than 95% as determined by SEC-HPLC.
Immobilized Human GDF15, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 67.1ng/ml determined by ELISA (QC Test).
Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag: Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.
Background
GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains amino acid residues a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
References
- Mullican S E, Lin-Schmidt X, Chin C N, et al. GFRAL is the receptor for GDF15 and the ligand promotes weight loss in mice and nonhuman primates[J]. Nature Medicine, 2017, 23(10):1150-1157.
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