Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein
Catalog No: GFRAL-CB001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Gln20-Glu351 (accession: XP_015304775.2).
Product Details
- Molecular Aliases
- GFR alpha-like; GFRAL; GRAL; C6orf144
- Protein Length
- Gln20-Glu351
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22μm filtered solution in 20mM PB, 400mM NaCl (pH 6.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Cynomolgus GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus GFRAL is greater than 90% as determined by SEC-HPLC.
Immobilized Cynomolgus GDF15, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 39.9ng/ml determined by ELISA (QC Test).
Background
GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains amino acid residues a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
References
- Mullican S E, Lin-Schmidt X, Chin C N, et al. GFRAL is the receptor for GDF15 and the ligand promotes weight loss in mice and nonhuman primates[J]. Nature Medicine, 2017, 23(10):1150-1157.
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