Biotinylated Human ENPP-3/CD203c (558-875) Protein
Catalog No: ENPP3-HB003
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human ENPP-3/CD203c (558-875) Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Lys558-Ile875 (UniProt accession: O14638).
Product Details
- Molecular Aliases
- E-NPP 3; NPP3; PD-Ibeta; NPPase; ENPP3; PDNP3; CD203c; B10; gp130RB13-6; PD-Iβ; PD-Ib; PD-I beta
- Protein Length
- Lys558-Ile875
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 39.14 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human ENPP-3 (558-875) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human ENPP-3 (558-875) is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human ENPP-3 (558-875), His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-ENPP3 Antibody, hFc Tag with the EC50 of 91.7ng/ml determined by ELISA. (QC Test)
Loaded Biotinylated Human ENPP-3 (558-875), His-Avi tag on Anti-His-Biosensor can bind Anti-ENPP-3 Antibody, hFc Tag with an affinity constant of 11.30 nM as determined in BLI assay.
Background
Ectonucleotide pyrophosphatase-phosphodiesterase 3 (ENPP3), a protein detected in the human uterus, has been found to play an important role in the development and invasion of tumours. It was recently discovered that ENPP3 was upregulated during the window of implantation in the human endometrium but its functional relevance remains elusive.
References
- Chen Q, Xin A, Qu R, Zhang W, Li L, Chen J, Lu X, Gu Y, Li J, Sun X. Expression of ENPP3 in human cyclic endometrium: a novel molecule involved in embryo implantation. Reprod Fertil Dev. 2018 Oct;30(10):1277-1285. doi: 10.1071/RD17257. PMID: 29614240.
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