Biotinylated Human CD5 Protein
Catalog No: CD5-HF003
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1), Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human CD5 Protein is expressed in HEK293 cells with an hFc (IgG1) tag and Avi tag at the C-terminus. It contains amino acid residues Arg25-Asn371 (UniProt accession: P06127).
Product Details
- Molecular Aliases
- CD5 molecule; CD5; LEU1; T1; CD5 antigen
- Protein Length
- Arg25-Asn371
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 67.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD5 is greater than 90% as determined by SEC-HPLC.
Immobilized Anti-CD5 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test).
Background
CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.
References
- Jevremovic, D., Dronca, R. S., Morice, W. G., McPhail, E. D., Kurtin, P. J., Zent, C. S., & Hanson, C. A. (2010). CD5 B-cell lymphoproliferative disorders: Beyond chronic lymphocytic leukemia and mantle cell lymphoma. Leukemia Research, 34(9), 1235-1238. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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