Human CD5 Protein
Catalog No: CD5-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human CD5 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Arg25-Pro372 (UniProt accession: P06127).
Product Details
- Molecular Aliases
- CD5 molecule; CD5; LEU1; T1; CD5 antigen
- Protein Length
- Arg25-Pro372
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 64.58 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD5 is greater than 95% as determined by SEC-HPLC.
Immobilized Human CD5, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CD5 Antibody, hFc-Avi tag with the EC50 of 11.0ng/ml determined by ELISA. (QC Test)
Background
CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.
References
- Jevremovic, D., Dronca, R. S., Morice, W. G., McPhail, E. D., Kurtin, P. J., Zent, C. S., & Hanson, C. A. (2010). CD5 B-cell lymphoproliferative disorders: Beyond chronic lymphocytic leukemia and mantle cell lymphoma. Leukemia Research, 34(9), 1235-1238. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.