Biotinylated Cynomolgus MADCAM1 Protein
Catalog No: MADCAM1-CB001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Cynomolgus MADCAM1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln22-Gln295 (accession: XP_045235080.2).
Product Details
- Molecular Aliases
- MAdCAM-1; MADCAM1; MACAM1
- Protein Length
- Gln22-Gln295
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 31.95 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Cynomolgus MADCAM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus MADCAM1 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Cynomolgus MADCAM1, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. (QC Test)
Background
Mucosal addressin cell adhesion molecule–1 (MAdCAM-1) is an endothelial cell marker that is recognized by its ligands on lymphocytes: L-selectin and α4β7 integrin. MAdCAM-1 is highly expressed on intestinal endothelium and has, recently, been shown to play an important role in the development of acute GVHD in animal model.
References
- Ambruzova Z, Mrazek F, Raida L, Stahelova A, Faber E, Indrak K, Petrek M. Possible impact of MADCAM1 gene single nucleotide polymorphisms to the outcome of allogeneic hematopoietic stem cell transplantation. Hum Immunol. 2009 Jun;70(6):457-60. doi: 10.1016/j.humimm.2009.03.008. Epub 2009 Mar 12. PMID: 19286444.
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