Biotinylated Cynomolgus IL-18 Protein
Catalog No: IL18-CB001
- Species
- Cynomolgus
- Expression System
- E.coli
- Tag
- an Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Cynomolgus IL-18 Protein is expressed in E. coli with an Avi tag at the C-terminus. It contains amino acid residues Tyr37-Asp193 (UniProt accession: A0A2K5TJ84).
Product Details
- Molecular Aliases
- IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1; IL1 gamma; IL1g; Interleukin-18; Interleukin 18; IL-1; IL 1; IL1
- Protein Length
- Tyr37-Asp193
- Expression System
- E.coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 20.34 kDa same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Cynomolgus IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus IL-18 is greater than 95% as determined by SEC-HPLC.
Immobilized Cynomolgus IL-18BP, His Tag (Cat. IL8-CM1BP) at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-18, Avi tag with the EC50 of 3.2ng/ml determined by ELISA. (QC Test)
Loaded Biotinylated Cynomolgus IL-18, Avi tag on Steptavidin-Biosensor can bind Cynomolgus IL-18 R1, His Tag (Cat. IL8-CM1R1) with an affinity constant of 4.11 nM as determined in BLI assay.
Background
Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
References
- Yamada T, Aoyama-Ishikawa M, Yamashita H, Fujiwara M, Usami M, Ueda T, Terashima M, Kohama K, Nakao A, Kotani J. IL-18 production and IL18 promoter polymorphisms correlate with mortality in ICU patients. In Vivo. 2014 May-Jun;28(3):391-6. PMID: 24815843.
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