Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (active dimer)
Catalog No: ALPP-CB001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Ile21-Thr503 (accession: XP_045223825.1).
Product Details
- Molecular Aliases
- Alkaline phosphatase; PLAP-1; ALPP; PLAP
- Protein Length
- Ile21-Thr503
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 53.69 kDa. Due to glycosylation, the protein migrates to 57-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl 2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Cynomolgus ALPP on Bis-Tris PAGE under reduced (R) condition and Non-reduced (N) condition. The purity is greater than 95%..
The purity of Biotinylated Cynomolgus ALPP is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Cynomolgus ALPP, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-ALPP Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. (QC Test)
Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Biotinylated Cynomolgus ALPP, His Tag with an affinity constant of 82.54 nM as determined in SPR assay (Biacore T200).
Loaded Anti-Alkaline Phosphatase Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus ALPP, His Tag with an affinity constant of 0.13 µM as determined in BLI assay.
Background
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
References
- Siller AF, Whyte MP. Alkaline Phosphatase: Discovery and Naming of Our Favorite Enzyme. J Bone Miner Res. 2018 Feb;33(2):362-364. doi: 10.1002/jbmr.3225. Epub 2017 Aug
- PMID:
- Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.