Cynomolgus GITR Ligand/TNFSF18 Protein
Catalog No: TNFSF18-CP001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Cynomolgus GITR Ligand/TNFSF18 Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Gln72-Ser199 (UniProt accession: A0A7N9CM47).
Product Details
- Molecular Aliases
- TNFSF18; AITRL; TL6; GITRL; GITR Ligand
- Protein Length
- Gln72-Ser199
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 15.62 kDa. Due to glycosylation, the protein migrates to 17-27 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Cynomolgus GITR Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus GITR Ligand is greater than 95% as determined by SEC-HPLC.
Cynomolgus GITR Ligand, His Tag immobilized on CM5 Chip can bind Cynomolgus GITR, His Tag with an affinity constant of 0.21 μM as determined in SPR assay (Biacore T200).
Background
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene. BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
References
- Bossen C, Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.
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