Biotinylated Human Claudin 4 Nanodisc
Catalog No: CLDN4-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Claudin 4 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val209 (UniProt accession: O14493).
Product Details
- Molecular Aliases
- Claudin-4; CLDN4; Claudin4; CPE-R; CPE R; CPETR1; WBSCR8
- Protein Length
- Met1-Val209
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.0 kDa.
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human Claudin 4 Nanodisc, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Claudin 4 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test).
Biotinylated Human Claudin 4 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-Claudin 4 Antibody, hFc Tag with an affinity constant of 6.145 nM as determined in SPR assay (Biacore T200).
Background
Claudin-4 (CLDN4) is a key component of tight junctions (TJs) in epithelial cells. CLDN4 is overexpressed in many epithelial malignancies and correlates with cancer progression. Changes in CLDN4 expression have been associated with epigenetic factors (such as hypomethylation of promoter DNA), inflammation associated with infection and cytokines, and growth factor signaling. CLDN4 helps to maintain the tumor microenvironment by forming TJs and acts as a barrier to the entry of anticancer drugs into tumors.
References
- Fujiwara-Tani R, Mori S, Ogata R, Sasaki R, Ikemoto A, Kishi S, Kondoh M, Kuniyasu H. Claudin-4: A New Molecular Target for Epithelial Cancer Therapy. Int J Mol Sci. 2023 Mar 13;24(6):5494. doi: 10.3390/ijms24065494. PMID: 36982569; PMCID: PMC10051602.
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