Biotinylated Human Transferrin R/CD71 Apical Domain Protein
Catalog No: CD71-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human Transferrin R/CD71 Apical Domain Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Lys189-Glu383 (UniProt accession: P02786).
Product Details
- Molecular Aliases
- TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR
- Protein Length
- Lys189-Glu383
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 24.96 kDa. Due to glycosylation, the protein migrates to 32-45 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human Transferrin R Apical Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Transferrin R Apical Domain is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human Transferrin R Apical Domain, His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. (QC Test)
Background
The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
References
- Jhee JH, Nam BY, Park JT, Kim HW, Chang TI, Kang EW, Lim BJ, Yoo TH, Kang SW, Jeong HJ, Han SH. CD71 mesangial IgA1 receptor and the progression of IgA nephropathy. Transl Res. 2021 Apr;230:34-43. doi: 10.1016/j.trsl.2020.10.007. Epub 2020 Oct 24. PMID: 33122053.
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