Biotinylated Cynomolgus GDF15 Protein (Primary Amine Labeling)
Catalog No: GDF15-CB001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Cynomolgus GDF15 Protein (Primary Amine Labeling) is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Ala197-Val308 (accession: G7PWZ3).
Product Details
- Molecular Aliases
- GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β; NAG1; RG1; Placental TGF-b; PTGF-b; PTGFBPTGF-b; Growth differentiation factor-15; Growth differentiation factor 15
- Protein Length
- Ala197-Val308
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Cynomolgus GDF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus GDF15 is greater than 95% as determined by SEC-HPLC.
Immobilized Cynomolgus GFRAL, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Cynomolgus GDF15, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA. (QC Test)
Immobilized Anti-GDF15 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GDF15, hFc Tag with the EC50 of 10.7ng/ml determined by ELISA.
Background
Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.
References
- Luan HH, Wang A, Hilliard BK, Carvalho F, Rosen CE, Ahasic AM, Herzog EL, Kang I, Pisani MA, Yu S, Zhang C, Ring AM, Young LH, Medzhitov R. GDF15 Is an Inflammation-Induced Central Mediator of Tissue Tolerance. Cell. 2019 Aug 22;178(5):1231-1244.e11. doi: 10.1016/j.cell.2019.07.033. Epub 2019 Aug 8. PMID: 31402172; PMCID: PMC6863354. Copy
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