Biotinylated Human GDF15 Protein
Catalog No: GDF15-HB001
- Species
- Human
- Expression System
- E.coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human GDF15 Protein is expressed in E. coli with a His tag and Avi tag at the N-terminus. It contains amino acid residues Ala197-Ile308 (UniProt accession: Q99988-1).
Product Details
- Molecular Aliases
- GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β; NAG1; RG1; Placental TGF-b; PTGF-b; PTGFBPTGF-b; Growth differentiation factor-15; Growth differentiation factor 15
- Protein Length
- Ala197-Ile308
- Expression System
- E.coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 15.19 kDa same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in 50 mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in 50mM HAc (pH 2.9). Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
Immobilized Biotinylated Human GDF15, His-Avi tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA. (QC Test)
Immobilized Biotinylated Human GDF15, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.
Background
Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.
References
- Baek SJ, Eling T. Growth differentiation factor 15 (GDF15): A survival protein with therapeutic potential in metabolic diseases. Pharmacol Ther. 2019 Jun;198:46-58. doi: 10.1016/j.pharmthera.2019.02.008. Epub 2019 Feb 18. PMID: 30790643; PMCID: PMC7196666.
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