Biotinylated Human B7-H2/ICOSLG Protein
Catalog No: ICOSLG-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human B7-H2/ICOSLG Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Asp19-Ser258 (UniProt accession: O75144).
Product Details
- Molecular Aliases
- CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7H2B7-related protein 1
- Protein Length
- Asp19-Ser258
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human B7-H2 is greater than 90% as determined by SEC-HPLC.
Immobilized Human ICOS (C136S,C137S), His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 25.1ng/ml determined by ELISA. (QC Test)
Loaded Biotinylated Human B7-H2, His-Avi tag on Streptavidin-Biosensor can bind Human ICOS (C136S, C137S), His Tag with an affinity constant of 77.40 nM as determined in BLI assay.
Background
B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
References
- Yao S, Zhu Y, Chen L. Advances in targeting cell surface signalling molecules for immune modulation[J]. Nature Reviews Drug Discovery, 2013, 12(2):130-146.
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