Human B7-H2/ICOSLG Protein
Catalog No: ICOSLG-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human B7-H2/ICOSLG Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Asp19-Ser258 (UniProt accession: O75144-1).
Product Details
- Molecular Aliases
- CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50;B7H2B7-related protein 1
- Protein Length
- Asp19-Ser258
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 53.4 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human B7-H2 is greater than 95% as determined by SEC-HPLC.
Immobilized Human ICOS (C136S, C137S), His Tag (Cat. ICO-HM102) at 5μg/ml (100μl/well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. (QC Test)
Immobilized Human ICOS, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA.
Human B7-H2 captured on Protein A chip, can bind Human ICOS (C136S,C137S), His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200).
Background
B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
References
- Yao S, Zhu Y, Chen L. Advances in targeting cell surface signalling molecules for immune modulation[J]. Nature Reviews Drug Discovery, 2013, 12(2):130-146. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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