Biotinylated Human GPRC5D Nanodisc
Catalog No: GPRC5D-HB004
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human GPRC5D Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val345 (UniProt accession: Q9NZD1-1).
Product Details
- Molecular Aliases
- GPRC5D; MGC129713; MGC129714; orphan G-protein coupled receptor
- Protein Length
- Met1-Val345
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 51.70 kDa.
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human GPRC5D Nanodisc, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate(5μg/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
Loaded Biotinylated Human GPRC5D Nanodisc, His Tag on Streptavidin-Biosensor can bind Anti-GPRC5D Antibody, hFc Tag with an affinity constant of 2.21 nM as determined in BLI assay.
Background
G protein–coupled receptor, family C, group 5, member D (GPRC5D) is an orphan receptor that is primarily expressed in plasma cells and hard keratinized tissues, with low expression in normal human tissues. GPRC5D expression is enriched in malignant plasma cells and is associated with markers of high-risk myeloma.
References
- Chari A, Minnema MC, Berdeja JG, Oriol A, van de Donk NWCJ, Rodríguez-Otero P, Askari E, Mateos MV, Costa LJ, Caers J, Verona R, Girgis S, Yang S, Goldsmith RB, Yao X, Pillarisetti K, Hilder BW, Russell J, Goldberg JD, Krishnan A. Talquetamab, a T-Cell-Redirecting GPRC5D Bispecific Antibody for Multiple Myeloma. N Engl J Med. 2022 Dec 15;387(24):2232-2244. doi: 10.1056/NEJMoa2204591. Epub 2022 Dec 10. PMID: 36507686.
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