Biotinylated Human IL-13 Protein
Catalog No: IL13-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-13 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Gly21-Asn132 (accession: AAK53823).
Product Details
- Molecular Aliases
- IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789; Interleukin-13; IL 13
- Protein Length
- Gly21-Asn132
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 15 kDa and 35-45 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated IL-13 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human IL-13 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human IL-13, His-Avi tag at 5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 0.81μg/ml determined by ELISA (QC Test).
Human IL-13Ra1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human IL-13, His Tag with an affinity constant of 25.59 nM as determined in SPR assay (Biacore T200).
Background
Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).
References
- Grünig G, Corry D B, Reibman J, et al. Interleukin 13 and the evolution of asthma therapy[J]. American Journal of Clinical & Experimental Immunology, 2012, 1(1):20.
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