Human IL-3 R alpha/CD123 Protein
Catalog No: CD123-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human IL-3 R alpha/CD123 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Thr19-Arg305 (UniProt accession: P26951-1).
Product Details
- Molecular Aliases
- CD123; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL3RAY; IL3RX; IL3RY; MGC34174; IL-3 R alpha; IL3R subunit alpha; IL3R alpha
- Protein Length
- Thr19-Arg305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 59.7 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result.
- Purity
- > 90% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IL-3 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Human IL-3 R alpha is greater than 90% as determined by SEC-HPLC.
Immobilized Human IL-3, Avi tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 44.8ng/ml determined by ELISA. (QC Test)
Immobilized Human IL-3 R alpha, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA.
Background
Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily. The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.
References
- Ugo Testa, Elvira Pelosi, Arthur Frankel. CD 123 is a membrane biomarker and a therapeutic target in hematologic malignancies. Biomark Res. 2014; 2: 4
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