Human IL-6 R alpha/CD126 Protein
Catalog No: CD126-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human IL-6 R alpha/CD126 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Leu20-Pro365 (UniProt accession: P08887-1).
Product Details
- Molecular Aliases
- IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1; IL6R; CD126; IL6RQ; gp80;IL6R subunit alpha; IL6R-alpha; IL6RA; IL6R1
- Protein Length
- Leu20-Pro365
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 65.3 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human IL-6 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-6 R alpha is greater than 90% as determined by SEC-HPLC.
Immobilized Human IL-6 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 6.0ng/ml determined by ELISA (QC Test).
Immobilized Human IL-6 R alpha, hFc Tag at 2μg/ml (100μl/well) on the Human IL-6, No Tag precoated plate (5μg/ml). Dose response curve for Human gp130, His Tag with the EC50 of 49.6ng/ml determined by ELISA.
Loaded Human IL-6 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-6, No Tag with an affinity constant of 16.50 nM as determined in BLI assay.
Background
Serum levels of interleukin-6 (IL-6) are increased in patients with type 2 diabetes (T2D). IL-6 exerts its pleiotropic effects via the IL-6 α-receptor (IL-6R), which exists in membrane-bound and soluble (sIL-6R) forms and activates cells via the β-receptor glycoprotein 130 (gp130).
References
- Aparicio-Siegmund S, Garbers Y, Flynn CM, Waetzig GH, Gouni-Berthold I, Krone W, Berthold HK, Laudes M, Rose-John S, Garbers C. The IL-6-neutralizing sIL-6R-sgp130 buffer system is disturbed in patients with type 2 diabetes. Am J Physiol Endocrinol Metab. 2019 Aug 1;317(2):E411-E420. doi: 10.1152/ajpendo.00166.2019. Epub 2019 Jun
- PMID:
- Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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