Biotinylated Human IL-18 Protein (Primary Amine Labeling)
Catalog No: IL18-HB001
- Species
- Human
- Expression System
- E.coli
- Tag
- Biotin
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human IL-18 Protein (Primary Amine Labeling) is expressed in E. coli without tag. It contains amino acid residues Tyr37-Asp193 (UniProt accession: Q14116-1).
Product Details
- Molecular Aliases
- IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1; IL-1γ; IL-1 γ; IL1 gamma; IL1g; IL1γ; IL1 γ; IL-1 g; Interleukin-18; Interleukin 18; IL-1; IL 1; IL1
- Protein Length
- Tyr37-Asp193
- Expression System
- E.coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 18.2 kDa same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20mM PB, 250mM NaCl, 50mM L-arginine (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-18 is greater than 90% as determined by SEC-HPLC.
Immobilized Human IL-18BP, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2 ng/ml determined by ELISA. (QC Test)
Human IL-18 R1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Biotinylated Human IL-18, No Tag with an affinity constant of 3.46 nM as determined in SPR assay (Biacore T200).
Background
Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
References
- Yamada T, Aoyama-Ishikawa M, Yamashita H, Fujiwara M, Usami M, Ueda T, Terashima M, Kohama K, Nakao A, Kotani J. IL-18 production and IL18 promoter polymorphisms correlate with mortality in ICU patients. In Vivo. 2014 May-Jun;28(3):391-6. PMID: 24815843.
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