Biotinylated Human GIPR Protein
Catalog No: GIPR-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human GIPR Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly26-Gln138 (UniProt accession: P48546-1).
Product Details
- Molecular Aliases
- GIP-R; GIPR; PGQTL2
- Protein Length
- Gly26-Gln138
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 15.88 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human GIPR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human GIPR is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human GIPR, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-GIPR Antibody, hFc Tag with the EC50 of 12.2ng/ml determined by ELISA. (QC Test)
Background
The gastric inhibitory polypeptide receptor (GIPR), a G protein-coupled receptor (GPCR) that regulates glucose metabolism and insulin secretion, is a target for the development of therapeutic agents to address type 2 diabetes and obesity.
References
- Brown KA, Morris RK, Eckhardt SJ, Ge Y, Gellman SH. Phosphorylation Sites of the Gastric Inhibitory Polypeptide Receptor (GIPR) Revealed by Trapped-Ion-Mobility Spectrometry Coupled to Time-of-Flight Mass Spectrometry (TIMS-TOF MS). J Am Chem Soc. 2023 Dec 27;145(51):28030-28037. doi: 10.1021/jacs.3c09078. Epub 2023 Dec 13. PMID: 38091482; PMCID: PMC10842860.
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