Biotinylated Human GIPR Nanodisc
Catalog No: GIPR-HB003
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human GIPR Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Cys466 (UniProt accession: P48546-1).
Product Details
- Molecular Aliases
- GIP-R; GIPR; PGQTL2
- Protein Length
- Met1-Cys466
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 63.8 kDa.
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
- State
- Liquid
- Storage Conditions
- Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Immobilized Biotinylated Human GIPR Nanodisc, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-GIPR Antibody, hFc Tag with the EC50 of 25.5ng/ml determined by ELISA (QC Test). (QC Test)
Biotinylated Human GIPR Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-GIPR Antibody, hFc Tag with an affinity constant of 18.70 nM as determined in SPR assay (Biacore T200).
Loaded Biotinylated Human GIPR Nanodisc, His Tag on Streptavidin-Biosensor can bind Anti-GIPR Antibody, hFc Tag with an affinity constant of 19.60 nM as determined in BLI assay.
Background
The gastric inhibitory polypeptide receptor (GIPR), a G protein-coupled receptor (GPCR) that regulates glucose metabolism and insulin secretion, is a target for the development of therapeutic agents to address type 2 diabetes and obesity.
References
- Brown KA, Morris RK, Eckhardt SJ, Ge Y, Gellman SH. Phosphorylation Sites of the Gastric Inhibitory Polypeptide Receptor (GIPR) Revealed by Trapped-Ion-Mobility Spectrometry Coupled to Time-of-Flight Mass Spectrometry (TIMS-TOF MS). J Am Chem Soc. 2023 Dec 27;145(51):28030-28037. doi: 10.1021/jacs.3c09078. Epub 2023 Dec 13. PMID: 38091482; PMCID: PMC10842860.
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