Biotinylated Human NKG2C/CD159c Protein
Catalog No: NKG2C-HB002
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human NKG2C/CD159c Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Glu98-Leu231 (UniProt accession: P26717).
Product Details
- Molecular Aliases
- CD159c; KLRC2; NKG2C; NK cell receptor C
- Protein Length
- Glu98-Leu231
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 35-55 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human NKG2C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Background
As a first line of defense, natural killer (NK) cells play a crucial role in the fight against infections. The presented study is the first of its kind that ascribes CD56dimCD16 NKG2C-expressing NK cells a crucial role in biasing adaptive immune responses upon influenza vaccination and suggests NKG2C as a potential biomarker in predicting pandemic influenza vaccine responsiveness.
References
- Riese P, Trittel S, Pathirana RD, Klawonn F, Cox RJ, Guzmán CA. Responsiveness to Influenza Vaccination Correlates with NKG2C-Expression on NK Cells. Vaccines (Basel). 2020 Jun 5;8(2):281. doi: 10.3390/vaccines8020281. PMID: 32517137; PMCID: PMC7349951.
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