Biotinylated Human NKG2C&CD94 Protein
Catalog No: NKG2C-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human NKG2C&CD94 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus of NKG2C and Flag tag at the N-terminus of CD94. It contains amino acid residues Glu98-Leu231(NKG2C) and Ser34-Ile179(CD94) (accession: P26717(NKG2C) & Q13241-1(CD94)).
Product Details
- Molecular Aliases
- NKG2C&CD94; NKG2C; CD94
- Protein Length
- Glu98-Leu231
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 15.3kDa (NKG2C)&17.9kDa (CD94). Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human NKG2C&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human NKG2C&CD94 is greater than 90% as determined by SEC-HPLC.
Background
NKG2C&CD94 is a C-type lectin heterodimer on NK cells and CD8+ cytotoxic T-cells, it can recognize peptides derived from the intracellular proteins in the context of HLA-E. NKG2C&CD94 itself has no signal transduction function but is an activating receptor on the surface of NK cells that involved in driving the NK-cell expansion.
References
- Pump WC, Kraemer T, Huyton T, Hò GT, Blasczyk R, Bade-Doeding C. Between Innate and Adaptive Immune Responses: NKG2A, NKG2C, and CD8⁺ T Cell Recognition of HLA-E Restricted Self-Peptides Acquired in the Absence of HLA-Ia. Int J Mol Sci. 2019 Mar 22;20(6):1454.
- Pump WC, Kraemer T, Huyton T, Hò GT, Blasczyk R, Bade-Doeding C. Between Innate and Adaptive Immune Responses: NKG2A, NKG2C, and CD8⁺ T Cell Recognition of HLA-E Restricted Self-Peptides Acquired in the Absence of HLA-Ia. Int J Mol Sci. 2019 Mar 22;20(6):1454.
- López-Botet M, Muntasell A, Vilches C. The CD94/NKG2C+ NK-cell subset on the edge of innate and adaptive immunity to human cytomegalovirus infection. Semin Immunol. 2014 Apr;26(2):145-51.
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