Biotinylated Mouse LY75/CD205 Protein (Primary Amine Labeling)
Catalog No: LY75-MB001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His
Product overview
Recombinant Biotinylated Mouse LY75/CD205 Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Ser28-Asp1667 (UniProt accession: Q60767).
Product Details
- Molecular Aliases
- Ly-75; DEC-205; CD205; CLEC13B; GP200-MR6; Ly75; DEC205; GP200MR6
- Protein Length
- Ser28-Asp1667
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 189.5 kDa. Due to glycosylation, the protein migrates to 200-250 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Mouse LY75 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Background
Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. In mammals, several cell surface molecular markers have been characterized in order to identify the mitotic germ cells. he expression profile of Ly75 protein was similar to that of the mRNA. Furthermore, identification of various fish homologs of ly75 confirmed that their amino acid sequences are well conserved. Therefore, Ly75 may be appropriate for use as a versatile cell surface marker for mitotic germ cells in fish.
References
- Nagasawa K, Shikina S, Takeuchi Y, Yoshizaki G. Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. Biol Reprod. 2010 Oct;83(4):597-606. doi: 10.1095/biolreprod.109.082081. Epub 2010 Jun 10. PMID: 20554922.
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