Biotinylated Human LY75/CD205 Protein (Primary Amine Labeling)
Catalog No: LY75-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human LY75/CD205 Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Ser28-Asp1666 (UniProt accession: O60449-1).
Product Details
- Molecular Aliases
- Ly-75; DEC-205; CD205; CLEC13B; GP200-MR6; Ly75; DEC205; GP200MR6
- Protein Length
- Ser28-Asp1666
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 190.2 kDa. Due to glycosylation, the protein migrates to 230-245 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human LY75 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated Human LY75, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. (QC Test)
Background
Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. In mammals, several cell surface molecular markers have been characterized in order to identify the mitotic germ cells. he expression profile of Ly75 protein was similar to that of the mRNA. Furthermore, identification of various fish homologs of ly75 confirmed that their amino acid sequences are well conserved. Therefore, Ly75 may be appropriate for use as a versatile cell surface marker for mitotic germ cells in fish.
References
- Nagasawa K, Shikina S, Takeuchi Y, Yoshizaki G. Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. Biol Reprod. 2010 Oct;83(4):597-606. doi: 10.1095/biolreprod.109.082081. Epub 2010 Jun 10. PMID: 20554922.
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