Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein
Catalog No: RIIB-HB001
- Species
- Human/Cyno
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus) (accession: Q13705-1(Human) / XP_045242398.1(Cynomolgus)).
Product Details
- Molecular Aliases
- Activin receptor type-2B; ACVR2B; Activin RIIB; ActRIIB
- Protein Length
- Ser19-Thr137
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 16.56 kDa. Due to glycosylation, the protein migrates to 33-43 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human/Cynomolgus Activin RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human/Cynomolgus Activin RIIB is greater than 95% as determined by SEC-HPLC.
Immobilized Human Activin A, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 53.8ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human/Cynomolgus Activin RIIB, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Activin RIIB Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
Biotinylated Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Activin A, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200).
Biotinylated Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via Streptavidin can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Biotinylated Human/Cynomolgus Activin RIIB, His Tag immobilized on CM5 Chip can bind Anti-Activin RIIB Antibody, hFc Tag with an affinity constant of 6.44 pM as determined in SPR assay (Biacore T200).
Background
ActRIIB (activin receptor type-2B) is an activin receptor subtype constitutively expressed in the whole body, playing a role in cellular proliferation, differentiation, and metabolism. For its various physiological activities, ActRIIB interacts with activin and multiple other ligands including myostatin (MSTN), growth differentiation factor 11 (GDF11), and bone morphogenetic protein 9 (BMP9).
References
- Sakamoto K, Kanematsu-Yamaki Y, Kamada Y, Oka M, Ohnishi T, Miwa M, Asami T, Inooka H. Identification of ligand-selective peptidic ActRIIB-antagonists using phage display technology. Biochem Biophys Rep. 2017 Jun 10;11:33-39. doi: 10.1016/j.bbrep.2017.06.001. PMID: 28955765; PMCID: PMC5614685.
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