Human/Cynomolgus Activin RIIB/ACVR2B Protein
Catalog No: RIIB-HF001
- Species
- Human/Cyno
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human/Cynomolgus Activin RIIB/ACVR2B Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ser19-Thr137 (Human) / Ser40-Thr158 (Cynomolgus) (accession: Q13705-1(Human) / XP_045242398.1(Cynomolgus)).
Product Details
- Molecular Aliases
- Activin receptor type-2B; ACVR2B; Activin RIIB; ActRIIB
- Protein Length
- Ser19-Thr137
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human/Cynomolgus Activin RIIB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human/Cynomolgus Activin RIIB is greater than 95% as determined by SEC-HPLC.
Immobilized Human/Mouse/Rat Activin A, No Tag (Cat. ACV-HM001) at 1μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).
Immobilized Human/Mouse/Rat GDF-8, No Tag (Cat. GDF-HM008) at 1μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 0.5ng/ml determined by ELISA.
Immobilized Human Latent GDF-11, His Tag (Cat. GDF-HM111) at 2μg/ml(100μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA.
Immobilized Human Activin B, No Tag (Cat. ACV-HM00B) at 2μg/ml (100μl/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
Immobilized Human/Cynomolgus Activin RIIB, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Activin RIIB Antibody, hFc Tag with the EC50 of 24.7ng/ml determined by ELISA.
Human/Cynomolgus Activin RIIB, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF-2, No Tag (Cat. GDF-HM002) with an affinity constant of 26.57 pM as determined in SPR assay (Biacore T200).
Loaded Human/Cynomolgus Activin RIIB, hFc Tag on ProA-Biosensor can bind Human Activin B, No Tag (Cat. ACV-HM00B) with an affinity constant of 0.19 nM as determined in BLI assay.
Loaded Human/Cynomolgus Activin RIIB, hFc Tag on ProA-Biosensor can bind Biotinylated Human/Mouse/Rat GDF-8, Avi tag (Cat. GDF-HM008B) with an affinity constant of 0.22 nM as determined in BLI assay.
Loaded Human/Cynomolgus Activin RIIB, hFc Tag on ProA-Biosensor can bind Human GDF-2, No Tag (Cat. GDF-HM002) with an affinity constant of 0.48 nM as determined in BLI assay.
Background
ActRIIB (activin receptor type-2B) is an activin receptor subtype constitutively expressed in the whole body, playing a role in cellular proliferation, differentiation, and metabolism. For its various physiological activities, ActRIIB interacts with activin and multiple other ligands including myostatin (MSTN), growth differentiation factor 11 (GDF11), and bone morphogenetic protein 9 (BMP9).
References
- Sakamoto K, Kanematsu-Yamaki Y, Kamada Y, Oka M, Ohnishi T, Miwa M, Asami T, Inooka H. Identification of ligand-selective peptidic ActRIIB-antagonists using phage display technology. Biochem Biophys Rep. 2017 Jun 10;11:33-39. doi: 10.1016/j.bbrep.2017.06.001. PMID: 28955765; PMCID: PMC5614685.
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