Human FGF4 Protein
Catalog No: FGF4-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human FGF4 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Ala31-Leu206 (UniProt accession: P08620-1).
Product Details
- Molecular Aliases
- FGF-4; FGF 4; HST; HST-1; HSTF-1; HBGF-4; KS3; FGF4; HST1; HSTF1; HBGF4; Fibroblast growth factor-4; Fibroblast growth factor 4
- Protein Length
- Ala31-Leu206
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 45.23 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human FGF4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human FGF4 is greater than 95% as determined by SEC-HPLC.
Immobilized Human FGFR3 alpha (IIIb), His Tag (Cat. FGF-HM43B) at 1μg/ml (100μl/well) on the plate. Dose response curve for Human FGF4, hFc Tag with the EC50 of 4.29μg/ml determined by ELISA. (QC Test)
Background
Fibroblast growth factor-4 (FGF-4), a highly mitogenic protein encoded by the k-fgf/hst oncogene, stimulates the growth of a variety of cells of mesenchymal and neuroectodermal origin. It is a member of the FGF family and plays several important roles in bone development during embryogenesis.
References
- Quito FL, Beh J, Bashayan O, Basilico C, Basch RS. Effects of fibroblast growth factor-4 (k-FGF) on long-term cultures of human bone marrow cells. Blood. 1996 Feb 15;87(4):1282-91. PMID: 8608216.
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